Okazaki fragments are short sequences of DNA nucleotides approximately to base pairs long in eukaryotes which are synthesized discontinuously and later linked together by the enzyme DNA ligase to create the lagging strand during DNA replication. During DNA replication, the double helix is unwound and the complementary strands are separated by the enzyme DNA helicase , creating what is known as the DNA replication fork. This causes periodic breaks in the process of creating the lagging strand. The primase and polymerase move in the opposite direction of the fork, so the enzymes must repeatedly stop and start again while the DNA helicase breaks the strands apart. Once the fragments are made, DNA ligase connects them into a single, continuous strand. Before this time, it was commonly thought that replication was a continuous process for both strands, but the discoveries involving E.
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