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PCV-2 genotype definition and nomenclature. Vet Rec ; Porcine circovirus diseases. Anim Health Res Rev ;6: Detection of porcine circovirus types 1 and 2 in serum and tissue samples of pigs with and without postweaning multisystemic wasting syndrome.
J Clin Microbiol ; Pathological, immunohistochemical, and in situ hybridization studies of natural cases of postweaning multisystemic wasting syndrome PMWS in pigs.
J Comp Pathol ; Quantification of porcine circovirus type 2 PCV2 DNA in serum and tonsillar, nasal, tracheo-bronchial, urinary and faecal swabs of pigs with and without postweaning multisystemic wasting syndrome PMWS. Vet Microbiol ; Effect of vaccination with selective bacterins on conventional pigs infected with type 2 porcine circovirus.
Vet Pathol ; Comparison of porcine circovirus type 2 load in serum quantified by a real time PCR in postweaning multisystemic wasting syndrome and porcine dermatitis and nephropathy syndrome naturally affected pigs. J Virol Methods ; Molecular characterization of Lelystad virus.
Porcine circovirus-2 and concurrent infections in the field. Prevalence of porcine reproductive and respiratory syndrome virus, porcine circovirus type 2 and porcine parvovirus from aborted fetuses and pigs with respiratory problems in Korea. J Vet Sci ; J Vet Diagn Invest ; Cytokine mRNA expression profiles in lymphoid tissues of pigs naturally affected by postweaning multisystemic wasting syndrome. J Gen Virol ; Cytokine profiles in peripheral blood mononuclear cells and lymph node cells from piglets infected in utero with porcine reproductive and respiratory syndrome virus.
Clin Diagn Lab Immunol ; Postweaning multisystemic wasting syndrome PMWS in pigs. A review. Vet Q ; Acta Vet Hung ; Clinical and pathological observations on pigs with postweaning multisystemic wasting syndrome. Comparison of RNA extraction methods for the detection of porcine reproductive and respiratory syndrome virus from boar semen. Porcine reproductive and respiratory syndrome virus infects mature porcine dendritic cells and up-regulates interleukin production.
Clin Vaccine Immunol ; J Immunol Methods ; PCR detection and evidence of shedding of porcine circovirus type 2 in boar semen. A proposal on porcine circovirus type 2 PCV2 genotype definition and their relation with postweaning multisystemic wasting syndrome PMWS occurrence.
Update on postweaning multisystemic wasting syndrome and porcine dermatitis and nephropathy syndrome diagnostics. Case-control study on the association of porcine circovirus type 2 and other swine viral pathogens with postweaning multisystemic wasting syndrome. J Virol ; Correlation between type of adaptive immune response against porcine circovirus type 2 and level of virus replication.
Viral Immunol ; Cytokine profiles of peripheral blood mononuclear cells from pigs with postweaning multisystemic wasting syndrome in response to mitogen, superantigen or recall viral antigens. Cytokine and C-reactive protein profiles induced by porcine circovirus type 2 experimental infection in 3-week-old piglets.
2012, Número 1
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DETECCIÓN DE CIRCOVIRUS PORCINO TIPO 2 EN CERDOS DEL ECUADOR MEDIANTE PCR
The diagnosis of PMWS is a difficult task and must follow three criteria: i the presence of compatible clinical signs, ii the presence of characteristic microscopic histopathological lesions, and iii the presence of PCV2 within these lesions in moderate or high. The presence of PCV2 in lymphoid tissues must be demonstrated by in situ hybridization or immunohistochemical methods. The in situ hybridization is a more complex and expense compared to other diagnostic tools, on the other hand, one problem concerning the immunohistochemical methods for PMWS diagnostic is the lack of a commercial anti-PCV2 peroxidase conjugate; therefore, the aim of this work was to obtain a polyclonal-antibody-immunoperoxidase-conjugate for the PCV2 specific detection. An anti-PCV2-peroxidase conjugated for the PCV2 specific detection was obtained based on the use of the available commercial vaccine against PCV2 as immunogenic inoculation for producing a polyclonal antibody in rabbits. The conjugate obtained was able to discriminate between PCV2 and PCV1 infections and a high sensitivity and specificity of the conjugate were observed.
[Circovirus and porcine circovirus infection: history of a controversy]